Study Characteristics of genotypes in gastric cancer cases and controls in the analysis of Interleukin-10 -592 Promoter Genetic Polymorphism.

#Data of cardia-subtype gastric cancer were accessible; ∧ Data of noncardia-subtype gastric cancer were accessible; * Data of sporadic diffuse-subtype gastric cancer were accessible; ¶ Data of intestinal-subtype gastric cancer were accessible. +Data of the status of Helicobacter pylori of gastric cancer were accessible. ⊿Here the ancestry of predominant participants in this study is annotated as Spanish ethnicity, which should be treated as Latinos rather than Caucasians [29]. RFLP: Restriction fragment length polymorphisms; TaqMan: 5′nuclease polymerase chain reaction assays; Pyrosequencing: a method of DNA sequencing (determining the order of nucleotides in DNA) based on the “sequencing by synthesis” principle. It differs from Sanger sequencing, in that it relies on the detection of pyrophosphate release on nucleotide incorporation, rather than chain termination with dideoxynucleotides; Direct sequencing: method of methylation analysis using bisulfite-treated DNA utilized PCR and standard dideoxynucleotide DNA sequencing to directly determine the nucleotides resistant to bisulfite conversion; ASP: the allele specific primer–polymerase chain reaction (ASP-PCR) method; Snapshot: the Snapshot assay which provides detection of certain SNPs.