S. novakii assembly annotation

The library was sequenced on a MinION R9.4.1 Flow Cell with approximately 2.6 Gb of data before filtering. Super accurate base-calling of the resulting FAST5 sequencing files was performed with Guppy v6.4 software provided by the company. The genome was assembled by using the FASTQ files after base-calling with the LRSDAY v1.6 pipeline combined with other software. After adapter trimming and filtering, Flye v2.9 was used for assembly. Long-read polishing was performed with Racon v1.4 and Medaka v1.4. The LRSDAY pipeline was also used to annotate the genome (using MAKER).