Pseudotime reconstruction of cytokinesis - examples

These two dataset represent a subset of the raw data that was acquired for pseudotime reconstruction of the intercellular bridge development in 6 cytokinetic steps. The data are fluorescence images of expanded NRK49F cells, undergoing cytokinesis. The images were acquired as z-stacks with 1 µm spacing and a x/y-pixel size of 90 nm on a spinning disk confocal microscope. Septin2-GFP, tubulin, a variable target and DAPI are visualized in 4 channels. The dataset is related to Zach Marin's 'Cytokinesis Pseudotime Analysis' package https://github.com/zacsimile/cytokinesis-pseudotime-analysis In order to test run the code, two different targets (MKLP1 and actin) are provided in /...example and /...example_2. Each target includes images of 6 differet stages (RC, CS, RS, SM, BA, A). The included xlsx-files provide the necessary information (length, X, Y) for aligning the intercellular bridges. A more detailed instruction for the use of this datset can be found on the Github page for 'Cytokinesis Pseudotime Analysis'.