Expression profiling of ncRNAs with custom microarray of CaV 1.3 knockout mice compared to wild type mice

For screening mouse models for CNS diseases for changes in ncRNA expression, we first investigated two models with impaired voltage-gated Ca2+ channel activity, i.e. the lethargic mutant of the auxiliary calcium channel beta4 subunit (Cacnb4lh; (Burgess et al. 1997)) and knockout mice for the L-type calcium channel CaV1.3 (Platzer et al. 2000), which have been implicated in a variety of neurological disorders such as psychiatric disorders (Cacnb4) or Parkinsons disease (Cav1.3).为筛选可用于中枢神经系统（Central Nervous System, CNS）疾病研究的小鼠模型，以探究其非编码RNA（non-coding RNA, ncRNA）表达变化，我们首先研究了两种电压门控Ca²+通道（voltage-gated Ca²+ channel）功能受损的小鼠模型：分别为辅助钙通道β4亚基嗜睡突变体（Cacnb4lh；Burgess等，1997），以及L型钙通道CaV1.3基因敲除小鼠（Platzer等，2000）。上述两种模型均与多种神经系统疾病相关，其中辅助钙通道β4亚基（Cacnb4）相关的突变体模型对应精神障碍，而L型钙通道CaV1.3相关的敲除模型则与帕金森病（Parkinson's disease）相关。