RNA-seq (Nova2-cKO, Nova2-KO, Nova2.Ptbp2-dKO)

We sequenced RNA from adult mouse spinal cord and from cell sorted Nova2-cKO neurons (crossed with Emx1-Cre, Gad2-Cre, Pcp2-Cre driver mice) and from Nova2.Ptbp2-dKO to compare gene expression level, and alternative splicing events. Neuronal cell-type specific mRNA profiles by deep sequencing using Illumina Hiseq2500.我们对成年小鼠脊髓、经细胞分选的Nova2条件性敲除（Nova2-cKO）神经元（与Emx1-Cre、Gad2-Cre、Pcp2-Cre驱动小鼠杂交获得）以及Nova2与Ptbp2双条件性敲除（Nova2.Ptbp2-dKO）样本的RNA进行了测序，以比较基因表达水平与可变剪接事件。本研究采用Illumina Hiseq2500平台开展深度测序，获取神经元细胞类型特异性的mRNA表达谱。