Single-cell Structure Probing of RNA Transcripts (sc-SPORT) of transfected RNAs in HEK293T cells

Here, we developed a new method- Single Cell Structure Probing Of RNA Transcripts scSPORT to simultaneously determine RNA secondary structural signal and gene expression in a single cell. To test this method, we introduced the wildtype versions of three different RNA Tetrahymena ribozyme, dengue 3UTR and HIV RNA, into a population of HEK293T cells and separately introduced the structure mutation versions, with a few disrupted pared bases, into a different population of cells.