Expression data of the human multiple myeloma cell line MM1.S after AKT inhibition in a FOXO3 deficient background

Forkhead box O transcription factors are important downstream targets of AKT signalling. In the human Multiple Myeloma celline MM1.S, AKT inhibtion leads to cell death in a FOXO3 dependent fashion. We used microarrays to asses which genes are up- or downregulated by FOXO3 after AKT inhibition by MK2206. Crispr Cas9 generated FOXO3 KO or Cas9 only Control clones were generated using the pl-CRISPR.EFS.GFP vector and subsequently sorted (GFP positive) and testing FOXO3 expression by Western Blot. 2 cas 9 only and 2 FOXO3 KO clones were treated O/N with an AKT inhibitor after which gene expression was analyzed.