Systemic environment in acute myocardial infarction programs human macrophages for trauma repair

In this study human primary monocyte-derived macrophages from healthy volunteers (pool of 6-8 donors) were exposed for 24 h to a 1:5 dilution of human patient serum. Patient sera were obtained either from control patients (n=20) or patients suffering an acute myocardial infarction (AMI, n=47, selected from POSTEMI trial, S. Limalanathan et al. J Am Heart Assoc 2014). All exposed macrophages were analyzed by RNA sequencing. The study revealed that macrophages significantly alter their gene expression profile in response the AMI-serum exposure, and adapt phagocytic, metabolic and cell cycling programs. Overall design: Primary monocytes were isolated from human PBMCs by CD14+ beads and differentiated into macrophages using recombinant human M-CSF 100ng/ml for seven days with one medium change. Patient or control serum was added directly into the growth medium of fully differentiated macrophages and incubated for 24h. Cells were washed, lysed and RNA sequencing was performed.