Barley and Arabidopsis NLR Alignment

Alignment of amino acid sequences of NB-ARC domains from barley NLRs predicted by NLR-parser and 15 known Arabidopsis NLRs, aligned by Clustal Omega. This alignment was used to generate the phylogenetic tree in Figure 4 in Carter and Helm, et al 2018 (in submission).<br>From the methods section:For the analysis of the barley and Arabidopsis NLRs, barley NLR genes for the entire Morex genome were predicted by NLR-Parser using the high confidence protein predictions (Steuernagel et al., 2015; Mascher et al., 2017). The amino acid sequence of NB-ARC domains identified in this pipeline were extracted into a FASTA file, to which the NB-ARC domains from 15 Arabidopsis CC-NB-LRRs were added from UniProt accessions. The combined 319 sequences were aligned using Clustal Omega<br>