Expression data from mouse lung epithelial cells during E. coli pneumonia in the presence or absence of LIF neutralization (6 h). Expression data from mouse lung epithelial cells during E. coli pneumonia in the presence or absence of LIF neutralization (6 h)

Leukemia inhibitory factor (LIF) is a cytoprotective cytokine that reduces lung injury during pneumonia. The purpose of this study was to determine the influence of LIF on pneumonia-induced gene changes in lung epithelium. Mice received intratracheal instillations of vehicle (PBS) or Escherichia coli (10^6 CFU). Control IgG or anti-LIF specific IgG was included in the instillate. 6 hours after the challenge, lung epithelial cells (EpCAM+/CD45-) were sorted from enzymatic lung digests and used for RNA isolation and microarray analysis. Overall design: RNA from 3 separate groups of mice (3 mice per group) was analyzed: 1) 6h Vehicle/control IgG-treated; 2) 6h E. coli/control IgG-treated; and 3) 6h E. coli/anti-LIF-specific IgG.