Offspring renal function is unaffected by maternal salt-exposure.

A 24 h urine collection with paired blood sample enabled analysis of renal function in offspring. Osmolarity and electrolytes were measured by an osmometer (Osmomat 030, Gonotec) and ICP-MS (XSeries II, Thermo Fisher, Ltd), respectively. Data are estimated marginal means plus the standard error of the differences between means (s.e.d.) for: Control diet (−ve salt, n = 7 dams), n = 13/13 male/female offspring; 4% salt (+ve salt, n = 5 dams), n = 6/8 male/female pups. Data were analysed by mixed effects models with treatment (control vs. 4% salt) and sex (male vs. female) or their interaction as fixed effects and dam included as a random term (Genstat v14). NS, not significant.