Gene expression changes in human pluripotent and fibroblast cells and mouse embryonic fibroblasts upon NORAD lncRNA knockdown

RNA sequencing data from knockdowns of the long non-coding RNA NORAD in human foreskin fibroblasts (HFFs), human embryonic stem cells (hESCs), and mouse embryonic fibroblasts (MEFs) for varying durations (8–72 hours, depending on the cell type). Total RNA was purified using TRI-reagent, and libraries were prepared using the CEL-seq2 protocol. RNA-seq data were processed with cutadapt, aligned to the reference genome using RNA-STAR, and gene expression counts were generated with HTSEQ count. Human embryonic stem cells, human foreskin fibroblasts, and mouse embryonic fibroblasts were transfected with siRNA against NORAD lncRNA and compared to control siRNA. Transfected cells were incubated for different time periods and then subjected to RNA sequencing. Each experiment was performed in several replicates.