Numerical data from: Gasotransmitter modulation of hypoglossal motoneuron activity

Using the rhythmic brainstem slice preparation, which contains the preBötzinger complex (preBötC) and the hypoglossal nucleus, we tested the hypothesis that central HO-2 dysregulation weakens hypoglossal motoneuron output. Disrupting HO-2 activity increased transmission failure as determined by the intermittent inability of the preBötC rhythm to trigger output from the hypoglossal nucleus.  Failed transmission was associated with a reduced input-output relationship between the preBötC and the motor nucleus.  These network phenomena were related to smaller inspiratory drive currents and reduced intrinsic excitability among hypoglossal neurons. In addition to HO-2, hypoglossal neurons also expressed the CO-regulated H2S producing enzyme cystathionine ϒ-lyase (CSE). H2S abundance was higher in hypoglossal neurons of HO-2 null mice than wild-type controls. Disrupting CSE function normalized transmission in HO-2 null mice and an H2S donor mimicked the effects of HO-2 dysregulation. These fin..., These data were collected from electrophysiological measurements.  Data was analyzed posthoc using CLAMPFIT, a part of the pCLAMP software suite (Molecular Devices).  Numerical values were generated using spreadsheet calculations in Excel (Microsoft). The resulting values were aggregated in a single summary file for each figure.  , Data is archived in a Microsoft Excel format.Â