Supplementary tables used in this study.

Table S1. ImageStream raw data used in Fig. 1. Table S2. DESeq2 results of RLRb high versus RLRb low immunostaining shown in Fig. 1F. Table S3. GSEA results shown in Fig. 1G. Table S4. DESeq2 results of mCherry positive versus mCherry negative sorted cells. Used in Fig. 3 H-j. Table S5. GSEA results shown in Fig. 3J. Table S6. Single-cell RNA-seq quality metrics and metacell expression matrix. Sheet “QC_metrics” contains CellRanger run summary statistics for the two biological replicates (Nvec01 and Nvec02), including cell numbers, sequencing depth, mapping rates, and RNA capture metrics. Sheet “Metacell_UMIs” contains normalized UMI fractions for each gene across all metacells and was used to generate the metacell-level expression profiles shown in Fig. 5B.. Table S7. Differential expression analysis of poly(I:C) versus NaCl treated embryos using MAST on scRNAseq data. Table S8. Marker genes reported by Cole et al. (48) Table S9. Cell cluster markers identified in this study. Used in fig. S6. Table S10. MAST differential expression for cluster 1 and GSEA results. Table S11. WGCNA analysis for identifying gene modules (fig. S7). Table S12. ORA results for GS17 (brown) gene module. Table S13. ORA results for GS16 (lightcyan) gene module. Table S14. ORA results for GS14 (red) gene module. Table S15. ORA results for GS15 (purple) gene module. Table S16. Marker genes identified in sub-clusters 0-3. Used in Fig. 6. Table S17. ORA results for sub-cluster 0. Table S18. ORA results for sub-cluster 1. Table S19. ORA results for sub-cluster 2. Table S20. ORA results for sub-cluster 3. Table S21. Orthofinder output used for orthogroups identification in Fig. 7. Table S22. Shared single orthogroups and their gene expression used for correlation analysis in Fig. 7D. Table S23. Shared genes with adjusted p value Table S24. Genes that were shared between cluster in the non-injected control and the immune cluster in adult Nematostella identified by Cole et al. (48). Table S25. Dictionary for converting NVE gene models, NV2, and the gene models used in this study and their associated annotations.