DDX50 cooperates with STAU1 to effect stabilization of pro-differentiation RNAs [rna_seq_stability_ddx50]

Glucose, traditionally recognized as a primary energy source, has recently emerged as a key regulator of protein interactions, particularly when intracellular glucose levels increase during cellular differentiation. Recent research has unveiled the intriguing phenomenon of glucose binding to a variety of proteins. Here, we focus on DDX50, a pivotal RNA helicase with a crucial role in epidermal differentiation. The interaction between glucose and the ATP-binding domain of DDX50 induces a significant change in the protein's conformation, resulting in the dissociation of DDX50 dimers. In the context of cellular differentiation, the increase in glucose levels promotes the formation of a complex involving DDX50, RNA, and STAU1. This complex, in turn, influences RNA structures and functions as a stabilizing force for the mRNA of key pro-differentiation genes. These genes include TINCR, OVOL1, CEBPB, PRDM1 and JUN. These findings reveal a previously unrecognized mechanism by which glucose exerts its influence, demonstrating its remarkable capacity to modulate the dimerization and function of the RNA helicase DDX50, which plays a critical role in tissue differentiation. Effect of DDX50 on RNA stability. 12 samples, two biological replicates, each with its own shRNA control. Two shRNAs against DDX50 (#1 and #4). RNA levels assayed before or after 4 h of actinomycin D treatment. ***Raw data have been deposited in dbGaP (phs003766.v1.p1)***