Claudio R. Thoma, Alexandre Matov, Katrin L. Gutbrodt, Christian R. Hoerner, Zlatko Smole, Wilhelm Krek, Gaudenz Danuser (2011) CIL:26570, Homo sapiens, epithelial cell, retinal pigment epithelial. CIL. Dataset

Video shows the dynamics of microtubules (MTs) using a GFP fusion construct of EB3 (end binding protein 3) that transiently binds to growing MT plus ends, generating a punctate pattern of EB3-GFP comets throughout the cell. RPE-1 cells were infected with a retroviral vector to express EB3-GFP. Cells on round coverslips were transferred to a homemade holding device, and 600 µl of the appropriate medium was added. The video was acquired with a microscope (IX70 Delta Vision Spectris; Olympus), temperature-controlled at 37C, using a 60× NA 1.4 differential interference contrast (DIC) oil Plan-Apochromat objective, ex 470 em 520, and a camera (CoolSNAP HQ; Roper Industries) with an exposure time of 100 ms and a frame rate of .5 s. Acquisition software used was SoftWoRx version 3.3.4 (Applied Precision). This is the raw data file for Video 1 and a frame from this is shown in Fig 1E of J Cell Biol. 2010. 190: 991-1003.

本数据集展示了通过EB3（端结合蛋白3）的GFP融合结构，EB3蛋白暂时性地结合于生长中的微管（MTs）的末端，在细胞内形成一系列EB3-GFP彗星样点状图案的微管动力学。RPE-1细胞经逆转录病毒载体感染以表达EB3-GFP。将培养在圆形盖玻片上的细胞转移至自制的固定装置中，并加入600 µl的适宜培养基。视频使用显微镜（IX70 Delta Vision Spectris；Olympus）采集，温度控制在37°C，采用60× NA 1.4差分干涉对比（DIC）油镜Plan-Apochromat，激发波长470 nm，发射波长520 nm，并配备相机（CoolSNAP HQ；Roper Industries），曝光时间为100毫秒，帧速率为0.5秒。采集软件为SoftWoRx版本3.3.4（Applied Precision）。这是视频1的原始数据文件，其中一帧显示在《细胞生物学杂志》2010年第190卷的图1E中。