241127_4DHSI_Bovine_Heart_LeftAtrium_RFA

Objective Research of 4D hyperspectral imaging as a visualization method for cardiac tissue in a bovine model. The main goal is to discriminate between RFA lesions and healthy tissue. Materials & Methods A fresh heart extracted from a male bovine (age: 1 year and 2-3 months, weight: 110-130 kg, death time: 10:08, heart removal time: 10:33) was delivered on ice to the Orbeli Institute, and dissection started at 13:04. Pieces of tissue were extracted from the left atrium. One left atria tissue piece was used for executing several RFA lesions and imaging using Nuance FX Imaging System (model: N-MSI-FX, CRi, Woburn, MA, USA). RFA machine (EPT-1000 XP, Boston Scientific) was set up at 8 W power and 15 s duration. Although, in some thin parts of the tissue the ablation is interrupted before, as follows: Ablation Description: Sample 1 Lesion number Duration (s) 1 15 2 13 3 15 4 15 4D Hyperspectral Imaging (HSI) Images were taken using a lab-assembled light source consisting of a xenon lamp coupled to several filters (343, 351, 360, 370, 380 and 390 nm, 10 nm FWHM), which can be switched using a filter wheel. White light image was taken with white LED light. Two sets of cubes were acquired for the same sample: the first cubes set was acquired with the sample placed on a plate without any solution and, for the second set it was immersed in cold PBS together with ice. Cube sets are available in the respective folders: Cubes_Sample1 Cubes_Sample1_PBS_Immersed Hyperspectral images were taken with a Nuance FX Imaging System (Model: N-MSI-FX, CRi, Woburn, MA, USA). For each sample, several cubes were acquired under illumination with each of the wavelengths (filters) mentioned above, as described in the respective metadata files for each sample. Metadata_Sample1.csv Metadata_Sample1_PBS_Immersed.csv TTC Staining After HSI acquisition, the ablation on the samples were revealed with 2,3,5-Triphenyltetrazolium chloride (TTC) staining. TTC aqueous solution was prepared the same day at 1 % concentration. Two slices were cut from the center of each lesion (denoted as “a” and “b”), and immersed in TTC solution. After at least 20 minutes of incubation, slices were photographed together with scale, for estimating lesion size. TTC staining results are available in the respective folder: TTC_Sample1 (corresponding to both cube sets) Data Management This dataset has been organized and structured according to the following guidelines: https://doi.org/10.5281/zenodo.17176953