Single cell RNA-seq analysis of mature thymic epithelial cells

This study set out to assay the (polyA+) transcriptomes of single mature (MHCII high) mouse medullary thymic epithelial cells (mTEC). Following isolation by FACs, the transcriptomes of single mature mTEC was assayed using the Fluidigm C1 microfluidics platform and Illumina RNA-seq.本研究旨在测定单个成熟的（MHC II高表达）小鼠髓质胸腺上皮细胞（medullary thymic epithelial cells，缩写mTEC）的polyA+转录组（polyA+ transcriptome）。研究人员经荧光激活细胞分选（Fluorescence Activated Cell Sorting，缩写FACs）分离细胞后，采用Fluidigm C1微流控平台与Illumina RNA测序技术对单个成熟mTEC的转录组进行了检测。