Figure 5 raw data

Experimental and computational raw data presented in Figure 5.Figure 5. Rab5 activation wave spreads on membrane in presence of GAP. (A) Micrographs of CF488A-Rab5 activation wave spreading across the membrane. Scale bar is 20 µm. Times indicate relative duration after start of acquisition, not time after addition of GEF complex. (B) Fluorescence intensity time profile of the indicated area in A. (C) Kymograph of the indicated area in A and mean wave velocity. Wave velocity was determined from the slope of fluorescence increase in generated kymographs (N = 6). (D) Simulated Rab5 activation front with diffusion inclusion in the phenomenological model (see SI Appendix, Supplementary Text Eq. 6). (F) Solution in terms of the dimensionless distance x/√(D/a_2 ).