长柄双花木种群遗传结构及种群历史

【目的】分析长柄双花木种群遗传多样性和遗传结构,探讨其种群演化历史,加深对该物种进化过程的理解,为其遗传资源保护、开发利用提供理论基础。【方法】利用15对能获得高多态性扩增产物的SSR引物,采用TP-M13-SSR技术检测长柄双花木全分布区12个自然种群261株个体的遗传多态性,应用Gene Marker软件进行基因分型,FSTAT估算遗传参数,ARLEQUIN进行分子方差分析,STRUCTURE对所有个体进行Bayesian聚类分析,Bottleneck检测种群遗传瓶颈效应。【Objective】To analyze the genetic diversity and genetic structure of Disanthus cercidifolius var. longipes populations, explore their population evolutionary history, deepen the understanding of the evolutionary process of this species, and provide a theoretical basis for the conservation, exploitation and utilization of its genetic resources. 【Method】Fifteen pairs of SSR primers yielding high-polymorphic amplification products were utilized, and the TP-M13-SSR technique was applied to detect the genetic polymorphism of 261 individuals from 12 natural populations of Disanthus cercidifolius var. longipes across their entire distribution range. Genotyping was performed using Gene Marker software, genetic parameters were estimated with FSTAT, analysis of molecular variance was conducted via ARLEQUIN, Bayesian cluster analysis of all individuals was carried out using STRUCTURE, and genetic bottleneck effects of the populations were detected using Bottleneck.