Effect of FTO-KD on m6A modification in GH3 cell line [MeRIP-seq]

Growth hormone-secreting pituitary adenomas account for approximately 10% of endocrine pituitary tumors and are pathologically classified into densely granulated adenomas (DGGH) and sparsely granulated adenomas (SGGH). m6A has been reported to play an important role in tumorigenesis and development, but its potential role in the pathogenesis of these two subtypes has not yet been thoroughly investigated. Consequently, we performed MeRIP-seq analysis of pituitary adenomas and the GH3 cell line. We observed that the expression of FTO was significantly increased in SGGH, leading to a reduction in m6A modification and a disruption of desmosome organization.分泌生长激素的垂体腺瘤约占内分泌性垂体肿瘤的10%，病理上可分为致密颗粒型腺瘤（DGGH）与稀疏颗粒型腺瘤（SGGH）。已有研究表明，N6-甲基腺嘌呤（m6A）在肿瘤发生发展中发挥重要作用，但其在这两种亚型腺瘤发病机制中的潜在功能尚未得到全面深入的探究。为此，我们针对垂体腺瘤组织及GH3细胞系开展了MeRIP-seq（甲基化RNA免疫沉淀测序）分析。研究发现，FTO（脂肪量和肥胖相关蛋白）在稀疏颗粒型腺瘤中表达显著上调，进而导致m6A修饰水平降低，并破坏桥粒的组织结构。