scRNA-seq time course of cerebellar Nestin-expressing progenitors from neonatal mice with and without injury at birth

To investigate the molecular changes that NEP subpopulations endure upon injury and to determine whether specific populations of NEPs have a transient increase in the signaling pathways associated with injury induced cellular stress and ROS, we performed multiplexed scRNA-seq of NEPs (CFP+) isolated by fluorescence activated cell sorting (FACS) from Nes-CFP/+ transgenic neonates with and without irradiation at timepoints P1, P2, P3 and P5.