Corpus callosum bulk RNA sequencing of a cuprizone mouse model of demyelination

We used the cuprizone mouse model of multiple sclerosis to determine whether Bruton's tyrosine kinase (BTK) inhibition could attenuate disease-relevant changes to central nervous system gene expression. The corpus callosum was dissected from the brains of a cuprizone mouse model of demyelination. Bulk RNA sequencing was then performed. A subset of mice had been treated with an inhibitor of Bruton’s tyrosine kinase (BTK) after disease induction.