Additional file 1 of Combination of lyophilized adipose-derived stem cell concentrated conditioned medium and polysaccharide hydrogel in the inhibition of hypertrophic scarring

Additional file 1: Figure S1. The graphical abstract of ADSCC-CM combined with polysaccharide hydrogel: The concentration of therapeutic cytokines in stem cell-conditioned medium was elevated by lyophilization and rehydration. Simultaneously, the ionic molecules in the conditioned medium helped connect the short nanofibers in the polysaccharide hydrogel, forming a semi-solid drug reservoir for scarring’s remission. Figure S2. Freeze-drying of ADSC-CM: (A)Complete lyophilization of ADSC-CM in the tested volume of 10 ml and 15 ml. (B) Comparison of the ADSC-CM weight before and after freeze-drying.附加文件1：图S1。脂肪干细胞条件培养基（ADSCC-CM）复合多糖水凝胶的图形摘要：通过冻干与复溶工艺提升干细胞条件培养基中治疗性细胞因子的浓度。与此同时，条件培养基中的离子分子可辅助连接多糖水凝胶内的短纳米纤维，形成用于瘢痕缓解的半固态药物储库。图S2：脂肪干细胞条件培养基（ADSCC-CM）的冻干实验：(A) 10ml与15ml受试体积的ADSCC-CM实现完全冻干。(B) ADSCC-CM冻干前后的重量对比。