Estimates of the amount of neuromuscular transmitter released by motor nerve terminals at different temperatures in the Antarctic fish Pagothenia borchgrevinki

The number of quantal doses of acetylcholine released by a nerve to activate a muscle can be estimated by recording repeated responses (end plate potentials of EPPs) to nerve stimulation and calculating mean EPP amplitude and variance. Evoked EPPS were recorded over a range of temperatures (-2°C to 12°C) and was used to estimate the amount of neuromuscular transmitter released by motor nerve terminals by calculating the synaptic quantal number (number of synchronous miniature end plate currents) and its variation over a range of temperatures.通过记录神经刺激诱发的重复响应（终板电位（end plate potentials, EPPs））并计算平均终板电位幅值与方差，可估算出神经为激活肌肉所释放的乙酰胆碱（acetylcholine）量子剂量数目。本数据集在-2℃至12℃的温度区间内记录了诱发终板电位，并通过计算突触量子数（同步微小终板电流（miniature end plate currents）的数量）及其在各温度下的变化规律，用以估算运动神经末梢所释放的神经肌肉递质量。