Impact of Early Bisphenol A Exposure on Transcriptome and Chromatin Accessibility in Brain Oligodendrocytes of Juvenile Mice [ATAC-seq]

This study evaluates the neurodevelopmental effects of bisphenol A (BPA) on oligodendrocytes, the neural cells responsible for myelination in the central nervous system. The primary aim was to investigate the changes in transcriptome and chromatin compaction induced by early BPA exposure in oligodendrocytes isolated from 2-week-old juvenile mice. Overall design: OF-1 mice were exposed to BPA in utero starting from gestational day 7 and continued during lactation. BPA was administered at a concentration of 250 µg/mL in the mothers' drinking water, leading to an estimated exposure of 40 µg/kg/day. On post-natal day 16, brains were collected from 9 control mice (5 males, 4 females) and 10 BPA-exposed mice (5 males, 5 females). Brain cells were dissociated, and oligodendrocytes were isolated using magnetic cell sorting with anti-O4 antibodies. Chromatin from 50,000 cells of each sample (total = 19) was Tn5-tagmented, and DNA was extracted for sequencing (ATAC-seq). Additionally, RNA was extracted from the remaining cells of 4 samples per group (total = 16) for transcriptome analysis using RNA-seq.