Rnt1 KO Sc and Wild-type BMA64 Sc

Strain background is BMA64 with RNT1 gene deleted with the TRP marker Media is SD -Trp (synthetic dextrose minimal media missing tryptophan) Cells were grown until midlog and harvested at OD600=0.4; MAS5 (Statistical Algorithm) Scaling:All Probe Sets Target Signal 500;Normalization:All Probe Sets; Alpha1=0.04; Alpha2=0.06; Tau=0.015 Strain background is BMA64 with plasmid expressing TRP marker Media is SD -Trp (synthetic dextrose minimal media missing tryptophan) Cells were grown until midlog and harvested at OD600=0.4 ; MAS5 (Statistical Algorithm) Scaling:All Probe Sets Target Signal 500;Normalization:All Probe Sets; Alpha1=0.04; Alpha2=0.06; Tau=0.015菌株背景为BMA64，采用TRP标记敲除RNT1基因；培养基为SD-Trp（即缺乏色氨酸的合成葡萄糖基础培养基）；细胞培养至对数中期，于OD600=0.4时收集样本；采用MAS5（统计算法）进行信号标化：所有探针组目标信号值设定为500；归一化范围覆盖所有探针组；参数Alpha1=0.04、Alpha2=0.06、Tau=0.015。 菌株背景为BMA64，携带表达TRP标记的质粒；培养基为SD-Trp（即缺乏色氨酸的合成葡萄糖基础培养基）；细胞培养至对数中期，于OD600=0.4时收集样本；采用MAS5（统计算法）进行信号标化：所有探针组目标信号值设定为500；归一化范围覆盖所有探针组；参数Alpha1=0.04、Alpha2=0.06、Tau=0.015。