LncRNA SENCR promotes cell proliferation and progression in non-small-cell lung cancer cells via sponging miR-1-3p

Studies have found that lncRNA SENCR (lncSENCR) is closely related to the progress of human cancer. This study aimed to investigate the biological and clinical roles of lncSENCR in non-small cell lung cancer (NSCLC). RT-qPCR was used to detect the expression of lncSENCR and miR-1-3p in NSCLC. CCK-8 assay, Transwell assay and EDU staining were used to detect the effects of lncSENCR and miR-1-3p on the proliferation, invasion and migration of NSCLC cells. Target gene prediction and screening, luciferase reporter assay were used to validate downstream target genes of lncSENCR and miR-1-3p. Western blotting was used to detect the protein expression levels of CDK4 and CDK6. The tumor changes in mice were detected by <i>in vivo</i> experiments in nude mice. The expression levels of lncSENCR were increased in NSCLC, while the expression levels of miR-1-3p were reduced. Knockdown of lncSENCR inhibited the growth and metastasis of NSCLC. LncSENCR was found to regulate tumor growth through miR-1-3p. Knockdown of miR-1-3p reversed the inhibitory effect of sh-SENCR on the proliferation of NSCLC cells. Furthermore, lncSENCR regulated the expression of CDK4 and CDK6 by acting as a cavern of miR-1-3p in NSCLC. LncSENCR promotes cell proliferation and progression of NSCLC by regulating the expression of miR-1-3p, and CDK4 and CDK6 may be involved in the underlying mechanism of lncSENCR in NSCLC. NSCLC: Non-small cell lung cancer.