Fig. 1 Position of the eleven Danish strains within the B. anthracis phylogenetic tree based on whole-genome SNP analysis.

Figure 1. Position of the eleven Danish strains from cattle within the B. anthracis phylogenetic tree based on whole-genome SNP analysis. Minimum spanning tree based on 6596 chromosomal SNPs (A), 222 pXO1 SNPs (B) and 166 pXO2 SNPs (C). The 13 different canSNP groups are color-coded: C.Br.A1055 in white, B.Br.CNEVA in yellow, B.Br.001/002 and B.Br.Kruger in orange, A.Br.011/009 in light blue, A.Br.008/011 in blue, A.Br.WNA in dark blue, A.Br.005/006 in pink, A.Br.003/004 in red, A.Br.001/002 in green, A.Br.WNA in dark green, A.Br.Aust94 in brown and A.Br.Vollum in purple. The position of the 11 Danish isolates (in bold and underligned), the African IEMVT89 and 40 available whole genome-sequenced strains is marked. The length of each branch is proportional (logarithmic scale) to the number of SNPs identified between strains. Indicated in red are the position and name of some new or published SNPs specific to various canSNP groups: A05 (A.Br.005/006 group); A.Br.008 (A.Br.008/009 group); A08/D and A08/D1 (A.Br.008/011); A.Br.011 (A.Br.011/009); A.Br.009 (A.Br.WNA); A.Br.002 and A01 (A.Br.001/002 and A.Br.Ames); A02, A02/A, A02/B, A02/B1 (A.Br.001/002 subgroup A02); A.Br.001 and A01/A-DK (A.Br.Ames); A.Br.013, A.Br.015a, A.Br.15b, A.Br.026 (A.Br.Aust94). Based on a parsimony approach, the trees sizes are, respectively, 6730 (A), 227 (B) and 168 SNPs (C), i.e. containing approximately 1.9 (A), 2.2 (B) or 1.2 (C) % of homoplasia.The minimum spanning tree was drawn in BioNumerics version 6.6 (Applied Maths) using default settings.图1 展示了基于全基因组单核苷酸多态性（Single Nucleotide Polymorphism, SNP）分析构建的炭疽芽孢杆菌（Bacillus anthracis, B. anthracis）系统发育树中，11株牛源丹麦菌株的进化位置。本研究基于6596个染色体SNP（A）、222个pXO1质粒SNP（B）及166个pXO2质粒SNP（C）构建了最小生成树。13个不同的经典SNP（canSNP）分组以不同颜色标识：C.Br.A1055为白色，B.Br.CNEVA为黄色，B.Br.001/002与B.Br.Kruger为橙色，A.Br.011/009为浅蓝色，A.Br.008/011为蓝色，A.Br.WNA为深蓝色，A.Br.005/006为粉色，A.Br.003/004为红色，A.Br.001/002为绿色，另一A.Br.WNA分支为深绿色，A.Br.Aust94为棕色，A.Br.Vollum为紫色。11株丹麦分离株（以加粗并下划线标注）、非洲菌株IEMVT89以及40株已公开全基因组测序菌株的位置均已标记。各分支长度与菌株间鉴定到的SNP数量呈正比（采用对数刻度）。红色标注的为各经典SNP分组特异性的部分新发现或已发表SNP的位置与名称：A05（对应A.Br.005/006组）；A.Br.008（对应A.Br.008/009组）；A08/D与A08/D1（对应A.Br.008/011组）；A.Br.011（对应A.Br.011/009组）；A.Br.009（对应A.Br.WNA组）；A.Br.002与A01（对应A.Br.001/002及A.Br.Ames组）；A02、A02/A、A02/B、A02/B1（对应A.Br.001/002的A02亚组）；A.Br.001与A01/A-DK（对应A.Br.Ames组）；A.Br.013、A.Br.015a、A.Br.15b、A.Br.026（对应A.Br.Aust94组）。基于简约法分析，三棵树的总突变步数分别为6730（A）、227（B）与168个SNP（C），即分别包含约1.9%（A）、2.2%（B）或1.2%（C）的同塑性突变。本研究的最小生成树采用BioNumerics 6.6版本（Applied Maths公司）以默认参数绘制。