Genome-wide maps of deaminated CPDs (dCPDs) in yeast cells

Here, we describe a genome-wide map of uracil lesions arising form deaminated UV-induced cyclobutane pyrimidine dimers (CPDs) in yeast (Saccharomyces cerevisiae). Deaminated CPDs (dCPDs) were mapped at single nucleotide resolution across the yeast genome using the new dCPD-seq method in repair-deficient (rad14∆), G2/M-arrested (cdc13-1) yeast cells immediately after UV irradiation (0 hour) and following 6 hour (6hr), 24hr or 48hr of deamination in arrested, repair-deficient yeast cells. We used these data to analyze CPD deamination in different trinucleotide sequence contexts, yeast genes, transcription factor binding sites, and nucleosomes.本研究描述了酿酒酵母（Saccharomyces cerevisiae）内，紫外线诱导的环丁烷嘧啶二聚体（cyclobutane pyrimidine dimers, CPDs）脱氨基后形成的尿嘧啶损伤的全基因组图谱。研究人员采用新型dCPD-seq技术，对修复缺陷型（rad14∆）且处于G2/M期阻滞（cdc13-1）的酵母细胞，在紫外线照射后即刻（0小时），以及在上述阻滞状态下完成6小时（6hr）、24小时或48小时脱氨基作用后，对其全基因组范围内的脱氨基CPDs（dCPDs）进行了单核苷酸分辨率定位。本研究利用上述数据集，分析了不同三核苷酸序列背景、酿酒酵母基因、转录因子结合位点以及核小体中的CPD脱氨基过程。