CRISPR/Cas9 knockout of shell matrix protein 1 in the slipper-snail Crepidula atrasolea

Over the past decade, CRISPR/Cas9 has transformed molluscan research, resulting in the emergence of multiple species tailored for studying molluscan-specific novelties. However, significant barriers persist, preventing CRISPR/Cas9 from being used by more species to address their novelties. Challenges include efficient delivery of reagents to eggs, requiring genomics resources necessary for validating successful gene edits. Shell formation is a novelty that gastropods are well-suited for studying. The goal of the study is to lay the groundwork for using CRISPR/Cas9 to answer biomineralization questions in C. atrasolea. The slipper snail Crepidula atrasolea produces large eggs that are amenable to microinjection of reagents, and gene expression atlases were made for studying shell development. To this end, we established embryo culturing and RNP optimization methods, provided validation of gene editing using sequencing approaches, and demonstrated high-content imaging to validate gen..., , , # CRISPR/Cas9 knockout of shell matrix protein 1 in the slipper-snail Crepidula atrasolea

[https://doi.org/10.5061/dryad.b2rbnzsq5](https://doi.org/10.5061/dryad.b2rbnzsq5)

## Description of the data and file structure

This dataset comprises sequencing and imaging data that were obtained in the present pre-print. Four main datasets are provided, and their directory contents are as follows:

### 1. MiSeq 2x250 bp sequencing of *SMP1* Crispant and Uninjected controls

* `Miseq-raw-reads-June2024.zip`: Raw reads for sequencing of 1, 5, 10, 15, and 30 embryo *SMP1* Crispant and uninjected Control amplicons.
* `SMP1-1bro-neg-092223_S8_L001_R1_001.fastq.gz`: 1 embryo uninjected left reads.  
* `SMP1-1bro-neg-092223_S8_L001_R2_001.fastq.gz`: 1 embryo uninjected right reads.  
* `SMP1-1bro-pos-092223_S10_L001_R1_001.fastq.gz`: 1 embryo crispant left reads.  
* `SMP1-1bro-pos-092223_S10_L001_R2_001.fastq.gz`: 1 embryo crispant right reads.  
* `SMP1-5bro-neg-092223_S7_L001_R1_001.fastq.gz`: 5...,