Effect of existence or depletion of LIN28B on gene expression during tumor growth of PDAC cells

To investigate the effect of existence or depletion of LIN28B in PDAC growth , we established cell lines with or without LIN28B expression. We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 different cells.Additionally, we performed the whole exome sequencing of 14837T and 15376T to compare their differences 9 samples. RNA of 15376T, LIN28B KO 15376T and 14837T were harvested using Trizol reagent. 3 ug of total RNA is used for the construction of sequencing libraries by Hiseq 4000 PE150. Additionally, 3 samples. Genomic DNA of 15376T, 14837T and WT tail tissue were harvested. 1 ug of genomic DNA is used for the construction of sequencing libraries