Gene expression profile at single cell level of immune cells from 2153L GEM tumors upon different treatments. Gene expression profile at single cell level of immune cells from 2153L GEM tumors upon different treatments

To understand how the combination of ORIN1001 plus docetaxel promotes anti-tumor immunity, we performed single-cell RNA sequencing (scRNA-seq) of CD45+ cells from 2153L tumors. Our data found that combo induces CD8+ T cells that express high levels of T cell activation, cytotoxicity, and exhaustion genes. combo also increase the cDC1 and MHC-II high macrophages which exhibited higher M1-like macrophages scores. Overall design: CD45+ immune cells were isolated by fluorescence-activated cell sorting and analyzed using scRNAseq.