DNA methylation and gene expression changes during acquired resistance to ALK inhibitors (450k DNA methylation microarray)

To identify novel mechanisms of acquired resistance to ALK inhibitors, we established in vitro models of acquired resistance to ceritinib using H3122 cell. In this study, we examined genome-wide DNA methylation changes during acquired resistance to ceritinib. We also examined the heterogeneity of parental and resistant cells using single cell RNA--seq. Single cell RNA-seq data of 2 samples, H3122 parental cells and one ceritinib-treated resistant cells, were generated using 10X Genomics Single Cell Solution. Sequencing was performed in paired end reads (2x100 bp) using NextSeq 500 platform (Illumina).