Carcass decay inhibits denitrification indirectly by regulating the microbiota and physicochemical properties in a model water system

Animal carcass decomposition is involved in nitrogen (N) cycle in the aquatic ecosystems, e.g., denitrification, volatilization, and leaching. The denitrification process is a crucial part of the nitrogen cycle, helping to alleviate biological toxicity resulting from nitrate accumulation. However, it is still unknown whether and how cadaver decay affects the denitrifiers and their activity in river water.
In this study, six denitrifiers (encoding napA, narG, nirK, nirS, norB, and nosZ, respectively) and denitrifying activity were investigated in a model water system with nitrogen pollution from corpse decomposition using high-throughput sequencing, real-time quantitative PCR, and denitrifying enzyme activity (DEA) assay.
Cadaver decay increased the quantity and altered the composition of denitrifying microbiota. Multiple regression on distance matrices revealed that corpse decay had a significantly greater impact on the denitrification communities compared to temperature. To our surpris..., , , # Carcass decay inhibits denitrification indirectly by regulating the microbiota and physicochemical properties in a model water system

[https://doi.org/10.5061/dryad.0vt4b8h6z](https://doi.org/10.5061/dryad.0vt4b8h6z)

Paper: Carcass decay inhibits denitrification indirectly by regulating the microbiota and physicochemical properties in a model water system

Authors: Jiawei Yang, Qiaoling Yu, Wanghong Su, Sijie Wang, Xiaochen Wang, Qian Han, Huan Li

Description:

This README file describes the data package accompanying the above publication.

## Files:

1. AIC.xlsx: Includes a y variable and the best 7 x variables filtered based on  Akaike’s information criterion (AIC).
2. ALPHA.xlsx: Includes the alpha diversity (Observed OTUs, Chao1, ACE, Shannon, Simpson, Coverage) of six microbial communities (i.e., napA, narG, nirK, nirS, norB, and nosZ). C, control groups; E, experimental groups; 1-4, 23℃; 5-8, 26℃; 9-12, 29℃; 13-16, 32℃; 17-20, 35℃.
3. DEA.xlsx: Includes the denitrification en...