Combined transcriptome and translatome analyses reveal a role for tryptophan dependent auxin biosynthesis in the control of DOG1 dependent seed dormancy. Arabidopsis thaliana

Transcriptome and translatome analyses of 6 and 24 hours imbibed seeds dormant and non-dormant seeds of NILDOG1-Cvi with and without addition of the transcription inhibitor Cordycepin. NILDOG1-Cvi is the Ler WT containing an introgression of the Cvi accession on chromosome 5, which includes the DOG1 gene (Bentsink et al., 2006). Overall design: Gene expression of total and polysomal bound mRNA of 6 and 24 hours imbibed dormant and non-dormant NILDOG1-Cvi seeds in the presence of water or the tranacriptional inhibitor cordecypin. For each sample condition three replicates were used (two replicates were used for P_Cor_D24.CEL, P_Cor_ND24.CEL, P_Water_D24.CEL)