MiR-765 functions as a tumour suppressor and eliminates lipids in clear cell renal cell carcinoma by downregulating PLP2

MicroRNAs (miRNAs) expression profiles are widely investigated in the major cancers, but their expression in patients plasmas of cancers have not yet to be fully elucidated. We investigated expression profiles of miRNAs in patients preoperative/postoperative plasmas of clear cell renal cell carcinomas (ccRCCs) by using a miRNAs microarray platform which covers a total of 1,523 human miRNAs. Plasma samples were extracted from EDTA-K 2 tubes and centrifuged at 800g for 10 minutes at 40C to remove cells and centrifuged again at 12,000g for 15 minutes at 40C to remove cellular debris, and then stored in a deep freezer at -80°C. All plasmas were obtained within 4 hours after drawing blood and stored at -80°C for further RNA extraction. 5 patients plasma samples with ccRCC preoperatively and at day 7 postoperatively were used and expression profiles of miRNAs were screened by using a miRNA microarray platform.