miRNA for MACF1 regulates osteoblast differentiation
收藏NIAID Data Ecosystem2026-03-14 收录
下载链接：
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE202962
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资源简介：
MC3T3-E1 cells were transfected with short hairpin RNA (shRNA) specifically targeting the murine MACF1 lentivirus vector or with scrambled shRNA, and the stably transfected cell lines were selected using puromycin. After 15 days of selection, all cells were collected for further study. The collected MC3T3-E1 cells were subjected to sequencing by RiboBio Co., Ltd. (Guangzhou, China) for miRNA microarray analyses. Total RNA was harvested and quantified, and its quantity and purity were assessed using a K5500 Micro-Spectrophotometer (Beijing Kaiao Technology Development Co., Ltd., Beijing, China). Here, A260/A280 ≥ 1.5 and A260/A230 ≥ 1 indicated acceptable RNA purity and RNA integrity number (RIN) ≥ 7 (based on an Agilent 2200 RNA assay, Agilent Technologies, USA) indicated acceptable RNA integrity. Genomic DNA contamination was evaluated by gel electrophoresis. **Please note that the original raw data files have been lost and thus are not provided.***
创建时间：
2022-10-27
