Neuronal surface binding of MBP (10 µg/ml, 5 min) is abolished by MBP-acidic lipid pre-mixing.

MBP was pre-mixed with various lipids, the mixture was used for neuronal incubation, and subsequent MBP surface staining was performed to assess MBP surface binding (see Materials and Methods for details). PtdIns: phosphatidylinositol; PS: phosphatidylserine; PA: phosphatidic acid; GM1: monosialoganglioside; GD1a: disialoganglioside; PC: phosphatidylcholine; PE: phosphatidylethanolamine.a: No MBP surface staining observed.b: MBP surface binding observed.c: Not done.Neuronal surface binding of MBP (10 µg/ml, 5 min) is abolished by MBP-acidic lipid pre-mixing.