Abcb4 liver expression profiling

The multidrug resistance gene 2 (Mdr2 or Abcb4) encodes a P-glycoprotein (Pgp) - a phospholipid flippase - that is the murine orthologue of the MDR3 gene in humans. The MDR2 protein is principally expressed in the bile canalicular membrane of the liver. A homozygous disruption of Abcb4 causes absence of phospholipids from bile. This phospholipid deficiency results in liver injury. The Balb-Abcb4-/- mouse strain has been generated by introgressing the Abcb4 knockout from the fibrosis-resistant FVB/NJ (FVB.129P2-Abcb4tm1Bor/J) strain into the susceptible BALB/cJ background, by backcrossing FVB- Abcb4-/- mice in to fibrosis-susceptible BALB/cJ mice for 10 generations. cDNA expression profiling of mRNA from liver of four single male mutant mice against a pool of RNA from four wild type controls (n=8). For each mutant animal two technical replicates were performed including a dye swap experiment.多药耐药基因2（multidrug resistance gene 2，Mdr2或Abcb4）编码P-糖蛋白（P-glycoprotein，Pgp）——一种磷脂翻转酶，该蛋白是人类MDR3基因的小鼠同源物。Mdr2蛋白主要在肝脏的胆小管膜上表达。Abcb4基因纯合敲除会导致胆汁中磷脂缺失，这种磷脂缺乏可引发肝损伤。Balb-Abcb4-/-小鼠品系的构建方式为：从抗纤维化FVB/NJ（FVB.129P2-Abcb4tm1Bor/J）品系中获取Abcb4基因敲除等位基因，通过将FVB-Abcb4-/-小鼠与纤维化易感型BALB/cJ小鼠连续回交10代，将该等位基因导入易感型BALB/cJ遗传背景中。本研究以4只野生型对照小鼠的RNA混合池为参照，对4只雄性突变小鼠的肝脏信使RNA（mRNA）开展cDNA表达谱分析（n=8）。每只突变小鼠均设置2次技术重复实验，并包含染料互换实验。