Amino acid sequence alignment of the CS/DS modification enzymes in human (hs) and zebrafish (dr) constructed with Clustal Omega.

A, alignment of CS/DS 4-O sulfotransferases. B, CS/DS 6-O sulfotransferases. C, CS/DS 4-sulfate 6-O sulfotransferases. D, CS/DS 2-O-sulfotransferases and E, CS/DS epimerases. Identical amino acids are marked with red letters on yellow background, conserved residues are indicated by blue letters on light blue background and blocks of similar residues marked with black letters on green background. (PDF)