Supplementary Datasets S2 and S3. ADP-ribosylated peptides identified in three Af1521 Macro pulldown experiments from AvrRpm1-HA samples by the standard method (S2) and the 'triggering' method (S3).

During infection, the plant pathogenic bacterium Pseudomonas syringae translocates type 3 effector proteins into the host cell. The effector protein AvrRpm1 is an ADP-ribosyltransferases that ADP-ribosylates host proteins to subvert plant innate immunity. The aim of this experiment was to compare two proteomics methods with respect to identification of ADP-ribosylated Arabidopsis thaliana proteins and peptides from a transgenic line that inducibly expresses AvrRpm1.