RNA-seq experiment comparing the transcriptomes of mESC derived primary endothelial cells expressing different levels of TAZ-CAMTA1

TAZ-CAMTA1 (TC) is one of two fusion proteins known to define Epithelioid Hemangioendothelioma (EHE), a rare vascular sarcoma. It is thought that TC expression represents the initiating event in EHE development. We have developed a model for studying EHE through the differentiation of mESCs into primary endothelial cells, in which TC and GFP expression is induced by the addition of doxycycline. The purpose of this RNA-seq experiment was to determine transcriptomic differences between endothelial cells expressing TC or not. To this end, endothelial cells were treated with doxycycline for 24 hours to induce TC expression, then sorted by flow cytometry into GFP high, low and negative populations whereby GFP is a marker for TC expression. These three populations along with uninduced control endothelial cells were then subject to RNA-seq. Analysis involved comparing the transcriptomes of the TC high, low and negative endothelial cell populations to the uninduced controls.