Gene expression profiles in liver of F344 rat treated with chemical

The rodent carcinogenicity test requires extremely long test term and large examination cost. Therefore, now we need to develop a short term and low cost screening method. We already developed a chemical carcinogenicity short term screening method CARCINOscreen®. This study was performed to validate this screening method. We conducted 28 days-repeated dose experiments in male F344 rats with 4 carcinogens and 2 non-carcinogens, and the gene expression profiles in liver were measureed by custom oligo microarrays.啮齿类动物致癌性试验（rodent carcinogenicity test）往往试验周期极长、检测成本高昂。因此，亟需开发周期更短、成本更低的筛选方法。本研究团队已成功开发一种化学致癌性短期筛选方法CARCINOscreen®，本研究旨在对该筛选方法进行验证。我们选取4种致癌物与2种非致癌物，对雄性F344大鼠（F344 rats）开展28天重复染毒试验，并通过定制寡核苷酸微阵列（custom oligo microarrays）检测肝脏组织的基因表达谱。