Transcriptomic characteristics of quiescent cells in human fallopian tube epithelial organoids detected by a fluorescent membrane dye PKH26

We established fallopian tube epithelial organoids from human fallopian tube tissues and cultured them in the organoid culturing cocktail medium. To investigate the heterogeneity of proliferation in the fallopian tube epithelial organoids, we stained the fallopian tube epithelial cells by a fluorescent membrane dye PKH26. As culturing them, the intensity of PKH26 was reduced in accordance with the proliferative level of each cell. We sorted the organoid cells by FACS into PKH26-retained slow cycling cells and PKH26-reduced proliferative cells, and compared their transcriptomic characteristics by bulk RNA sequencing and gene set enrichment analysis (GSEA). The result of GSEA showed that several pathways related to cell cycle were downregulated in PKH26-retained cells. So this dataset is supposed to be suitable for the analysis of quiescent cells in human fallopian tube epithelium. Overall design: To investigate the characteristics of quiescent cells in fallopian tube epithelial organoids, we stained the fallopian tube epithelial cells with a fluorescent membrane dye PKH26. After culturing the organoids, we sorted the fallopian tube epithelial cells by FACS into PKH26-retained slow-cycling cells and PKH26-reduced proliferative cells. We compared the transcrioptomic characteristics by bulk RNA sequencing.