Adoptive transfer of immune splenocytes from CCR5+/+ or CCR5−/− donor mice protect CCR5-deficient recipient mice against Japanese encephalitis.

aEight-week-old donor CCR5+/+ or CCR5−/− mice were infected with 103 PFU of JEV, i.v., or left uninfected and sacrificed 7 days later for splenocyte collection with or without depletion of B cells. Cells were transferred to 8-week-old CCR5−/− recipient mice infected 3 days or 1 day earlier with 103 PFU of JEV i.v. Surviving mice were monitored for 28 days.bData are representative of 2 independent experiments. Immune splenocyte treatment groups were compared to the naive splenocyte control group to test for statistical significance.cNo significant difference was noted between immune splenocyte treatment and control groups.