Global transcriptional response in Japanese medaka larvae following embryonic exposure to TCDD. Oryzias latipes

A global RNA-Seq analysis was conducted using RNA isolated from the axial region of DMSO- and 0.3 nM TCDD-exposed medaka to identify targets within the osteochondral pathway potentially impacted by TCDD exposure. In total, 597 genes were significantly up- or down-regulated (q<0.05) and were associated with select pathological states including inflammatory disease, connective tissue disorders, and skeletal and muscular disorders. Overall design: RNA was isolated from axial tissue (containing vertebrae and surrounding muscle, fat, and dermis) from Japanese medaka larvae. Whole transcriptome libraries from DMSO and TCDD-medaka were generated for downstream RNA-Seq using the Ion Torrent PGM platform.