Complete sequencing of engineered chloroplast expression plasmids for bidirectional promoter analysis in Chlamydomonas reinhardtii.

This project aims to validate and document the complete sequences of five engineered plasmidsused for chloroplast genetic engineering in C. reinhardtii. The plasmids (pABDP1, pABDP2,pABDP3, pAMVe, and pATDt) contain natural bidirectional (pABDP1, pABDP2, and pABDP3)and monodirectional (pAMVe, and pATDt) promoter constructs designed to drive multigeneexpression in the chloroplast. Each plasmid was purified from E. coli and sequenced usingwhole-plasmid sequencing to confirm integrity, detect mutations, and ensure accurate annotationprior to functional experiments. The dataset includes the raw sequencing files and correspondingmetadata for each plasmid.