云杉属胚性组织超低温保存技术研究进展

简述了超低温保存植物细胞的基本原理；介绍了超低温保存植物材料的一般程序，包括材料选取、预处理、超低温冷冻、解冻复性培养的方法，重点介绍了两种常用超低温冷冻方法——程序降温冻存法和玻璃化法的操作步骤。其次，阐述了影响云杉属超低温保存的主要因素，包括：基因型、细胞年龄及生长状态，冷冻保护剂和各种预处理方法，不同超低温冷冻方法及降温速率。This overview briefly expounds the fundamental principles of cryopreservation for plant cells; it introduces the general procedures for cryopreserving plant materials, including material selection, pretreatment, cryogenic freezing, and thawing and recovery culture, with a particular focus on the operational steps of two commonly used cryopreservation methods: controlled-rate freezing and vitrification. Next, it elaborates on the main factors influencing the cryopreservation of Picea species, including genotype, cell age and growth status, cryoprotectants and various pretreatment methods, different cryopreservation methods as well as cooling rates.