Raw data of Cedar Creek Experiment

1.-Experiment 

The experimental design consisted of 21 populations inoculated with 1 mL water samples taken near the Cedar Bog Lake dock on July 7th. Each population consisted of a final volume of 39 mL in transparent Greiner Bio-One vented-cap culture flasks. We used a three-by-two factorial design with three nitrogen concentrations (standard BG11 medium (17.6 mM NaNO3), a reduced nitrogen BG11 medium (9 mM NaNO3), and a BG11-0 medium (without nitrogen)) and (10 mM NaCl) and without NaCl addition. To account for the effect of nutrient addition, we added a control treatment with deionized water. Three replicates population of each experimental condition were stabilized. The experiment was run for 3 weeks. 

Table 1: Experimental design. 

dH20

BG11

BG11 reduce Nitrogen

B11 without Nitrogen 

BG11 +NaCl

BG11 reduce Nitrogen

+NaCl

B11 without Nitrogen+NaCl

R1

R16

R10

R4

R19

R13

R7

R2

R17

R11

R5

R20

R14

R8

R3

R18

R12

R6

R21

R15

R9

1.1 FlowCam( Fluid Imaging Technologies) Data collection

0.15 mL aliquots of each population at week three were run through a Flowcam cytometer (Fluid Imaging Technologies) at a speed of 0.14 mL/min and collect images of individual particles and estimate biovolume using total area. The 10x magnification setting was used with a calibration factor of 0.5525. For each sample, the first 1,000 images taken by FlowCam were selected for species diversity analysis. 

Each folder is a separate population at T3 (21 days). It contains the original run (pictures and files) and within it can be found the classification by “groups” (pictures and files).

1.2 Flotation Device Technical Description

Flotation Device is a suspension system to safely maintain planktonic microbial populations to carry out studies at the water temperature and light conditions of a water body. 

1.3 Bradford Protein Assay Data 

Populations were checked weekly by sampling aliquots of all the populations and running Bradford Protein Assays as a proxy for population growth.